Automatic Object Counting In Neubauer Chambers

This paper presents a method to automate the object counting in Neubauer chambers. The proposed technique employs digital image processing to isolate the chamber grid markings and to identify each region of interest, which, in turn, enables the ability to perform the automatic counting for each region using the method that best suits the problem at hand. The technique’s implementation includes an interface that allows the selection and combination of multiple regions according to the needs of the experiment. The capabilities of the method are illustrated by tackling the difficult problem of counting spores of the Clonostachys rosea fungus.